Products
IGF1R antibody
- Product Name
- IGF1R antibody
- Catalogue No.
- FNab04170
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- insulin-like growth factor 1 receptor
- Alternative Names
- CD221 antibody, IGFIR antibody, IGFR antibody, JTK13 antibody
- UniProt ID
- P08069
- Observed MW
- 70 kDa,90-100 kDa, 130 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF
- Recommended dilution
- WB: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
MCF7 cells were subjected to SDS PAGE followed by western blot with FNab04170(IGF1R-Specific antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using FNab04170(IGF1R-Specific antibody) at dilution of 1:100
Immunofluorescence analysis of NIH/3T3 cells using FNab04170 (IGF1R-Specific antibody) at dilution of 1:100. Blue: DAPI for nuclear staining.
- Background
- IGF1R, also named as CD221, is a receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). IGF1R binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity, the activated IGF1R is involved in cell growth and survival control. It is crucial for tumor transformation and survival of malignant cell. IGF1R is a heterotetrameric receptor, composed of two alpha subunits (130 kDa each) and two beta subunits (~98 kDa each) (PMID: 6279656). Both the alpha and beta subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds.
